PDGFRA Antikörper

Produktnummer ABIN967626

Produktdetails anti-PDGFRA Antikörper

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse PDGF Receptor alpha chain

Klon: APA5

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody has been tested by immunofluorescent staining (Less or equal than 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-PDGFRA Antikörper (ABIN967626)

Fruttiger, Calver, Krüger, Mudhar, Michalovich, Takakura, Nishikawa, Richardson: "PDGF mediates a neuron-astrocyte interaction in the developing retina." in: Neuron, Vol. 17, Issue 6, pp. 1117-31, (1997) (PubMed).

Takakura, Yoshida, Kunisada, Nishikawa, Nishikawa: "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 770-7, (1996) (PubMed).

Heldin: "Structural and functional studies on platelet-derived growth factor." in: The EMBO journal, Vol. 11, Issue 12, pp. 4251-9, (1992) (PubMed).

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: CD140a (PDGFRA Produkte)

Hintergrund: The APA5 antibody reacts with Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140a, PDGFR-a), a receptor tyrosine kinase which is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells. PDGFR-a binds to PDGF A and B chains, in contrast to PDGFR-b, which binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Binding to PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The mAb APA5 has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
Synonyms: PDGF Receptor alpha chain

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling