BAK1 Antikörper

Produktnummer ABIN967519

Dieses Maus Monoklonal-Antikörper erkennt spezifisch BAK1 in WB. Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für BAK1 Antikörper (ABIN967519)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: G317-2

Produktdetails anti-BAK1 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Bak

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (1-2 µg/ml). HeLa cells (ATCC CCL-2) are recommended as a positive control. HeLa cell lysate is also available as a ready-to-use positive western blot control (ABIN968535).

Kommentare:

Related Products: ABIN967389, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-BAK1 Antikörper (ABIN967519)

Chittenden, Harrington, OConnor, Flemington, Lutz, Evan, Guild: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, (1995) (PubMed).

Kiefer, Brauer, Powers, Wu, Umansky, Tomei, Barr: "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 736-9, (1995) (PubMed).

Reed: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, (1994) (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, (1994) (PubMed).

Details zu BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Andere Bezeichnung: Bak

Hintergrund: Bak (for Bcl-2 homologous Antagonist/Killer) is a recently identified member of the Bcl-2 family. Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members, including Bcl-2, act as inhibitors of apoptosis, whereas others such as Bax promote cell death. Like Bax promote cell death. Like Bax, Bak primarily promotes apoptosis. However, unlike Bax, Bak has also been shown to inhibit cell death. Bak inhibited both serum-starvation and drug induced apoptosis when overexpressed in an Epstein-Barr virus (EBV)-transformed cell line. However, Bax did not inhibit cell death under the same conditions. Bak mNRA has been identified in a wide variety of fetal and adult tissues, with the highest levels observed in heart and skeletal muscle. The apparently ubiquitous expression of Bak has lead to the suggestion that its function may be mediated by cell death inhibitory factors, particularly in cell types with a long life span. Bak migrates at a reduced molecular weight ~24 kDa. G317-2 reacts with human Bak. A synthetic peptide corresponding to the first 50 amino acids of human Bak was used as immunogen.

Molekulargewicht: 24 kDa

Pathways: Apoptose, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response