PARP1 Antikörper (full length)

Produktnummer ABIN967515

Zitiert in 4+ Publikationen. Der Maus Monoklonal anti-PARP1 Antikörper (Klon C2-10) (ABIN967515) detektiert spezifisch PARP1 in WB und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Rind (Kuh) und Hamster.

Kurzübersicht für PARP1 Antikörper (full length) (ABIN967515)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Human, Maus, Rind (Kuh), Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: C2-10

Produktdetails anti-PARP1 Antikörper

Bindungsspezifität: full length

Kreuzreaktivität: Maus, Hamster, Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: Ascites

Immunogen: Cow PARP (full-length, thymus derived)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody is prepared as an ascites solution. For western blot analysis, investigators are highly encouraged to titrate the antibody for optimal performance. A titration range between 1:2000 to 1:4000 is suggested.

Kommentare:

Related Products: ABIN967540, ABIN967299, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-PARP1 Antikörper (ABIN967515)

Patel, Gores, Kaufmann: "The role of proteases during apoptosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 5, pp. 587-97, (1996) (PubMed).

Tewari, Quan, ORourke, Desnoyers, Zeng, Beidler, Poirier, Salvesen, Dixit: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, (1995) (PubMed).

Lamarre, Talbot, de Murcia, Laplante, Leduc, Mazen, Poirier: "Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study." in: Biochimica et biophysica acta, Vol. 950, Issue 2, pp. 147-60, (1988) (PubMed).

Lamarre, Talbot, Leduc, Muller, Poirier: "Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 64, Issue 4, pp. 368-76, (1986) (PubMed).

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP

Hintergrund: PARP [Poly(ADP-Ribose) Polymerase] is a 113 kDa nuclear chromatin associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular reponse to DNA damage and is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from a 113 kDa intact form into 89 kDa and 24 kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the C-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis. This antibody has been reported to recognize an epitope located within the DNA-binding domain of the enzyme.
Synonyms: Poly (ADP-Ribose) Polymerase

Molekulargewicht: 113 kDa (full-length), 89 kDa (cleaved)

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location