CD247 Antikörper (C-Term)

Produktnummer ABIN967446

Dieses Anti-CD247-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD247 in WB und IP. Geeignet für Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für CD247 Antikörper (C-Term) (ABIN967446)

Target: CD247 (CD247 Molecule (CD247))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD247 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 1zeta3A1

Produktdetails anti-CD247 Antikörper

Bindungsspezifität: C-Term

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CD3zeta C-terminus synthetic peptide

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells). Immunoprecipitation application is not routinely tested, only tested during development.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD247 Antikörper (ABIN967446)

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, (1993) (PubMed).

Details zu CD247

Target: CD247 (CD247 Molecule (CD247))

Andere Bezeichnung: CD247

Hintergrund: The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: gamma, delta, epsilon, zeta, and eta. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1zeta3A1 recognizes an epitope in the C-terminus of mouse CD3zeta. A synthetic peptide corresponding to the C-terminus of mouse CD3zeta (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Synonyms: CD3zeta chain

Molekulargewicht: 32 kDa

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway