ASK1 Antikörper

Produktnummer ABIN966515

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-ASK1 Antikörper wird verwendet zum Nachweis von ASK1 in Proben von Human. Er wurde validiert für WB, IHC, ELISA, IF und FACS.

Kurzübersicht für ASK1 Antikörper (ABIN966515)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ASK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 2E4

Produktdetails anti-ASK1 Antikörper

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-ASK1 Antikörper (ABIN966515)

Pressinotti, Klocker, Schäfer, Luu, Ruschhaupt, Kuner, Steiner, Poustka, Bartsch, Sültmann: "Differential expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low- and high-risk prostate cancer." in: Molecular cancer, Vol. 8, pp. 130, (2010) (PubMed).

Widenmaier, Ao, Kim, Warnock, McIntosh et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent ..." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, (2009) (PubMed).

Woolcott, SenGupta, Hanna, Aronson: "Estrogen and progesterone receptor levels in nonneoplastic breast epithelium of breast cancer cases versus benign breast biopsy controls." in: BMC cancer, Vol. 8, pp. 130, (2008) (PubMed).

Details zu ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Andere Bezeichnung: MAP3K5

Hintergrund: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun Nterminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Synonyms: ASK1, MEKK5, MAPKKK5

Molekulargewicht: 55kDa

Gen-ID: 4217

Pathways: MAPK Signalweg, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response