IGF1R Antikörper

Produktnummer ABIN966348

Produktdetails anti-IGF1R Antikörper

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reaktivität: Human

Wirt: Bitte anfragen

Klonalität: Monoklonal

Konjugat: Dieser IGF1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Aufreinigung: Crude ascites.

Immunogen: Ni-NTA purified truncated recombinant IGF1R-His expressed in E. Coli strain BL21 (DE3)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: -20 °C

Referenzen für anti-IGF1R Antikörper (ABIN966348)

Scheidegger, Cenni, Picard, Delafontaine: "Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects." in: The Journal of biological chemistry, Vol. 275, Issue 49, pp. 38921-8, (2001) (PubMed).

Fujimoto, Tomita, Wakamatsu, Tanimoto, Ike: "Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response." in: Journal of bacteriology, Vol. 177, Issue 19, pp. 5574-81, (1995) (PubMed).

Details zu IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Andere Bezeichnung: IGF1R (IGF1R Produkte)

Hintergrund: IGF1R(insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Pathways: RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie