GCK Antikörper

Produktnummer ABIN966192

Dieses Anti-GCK-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GCK in WB und ELISA. Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für GCK Antikörper (ABIN966192)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GCK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 4G6

Produktdetails anti-GCK Antikörper

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human GCK expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-GCK Antikörper (ABIN966192)

Barbetti, Cobo-Vuilleumier, Dionisi-Vici, Toni, Ciampalini, Massa, Rodriguez-Bada, Colombo, Lenzi, Garcia-Gimeno, Bermudez-Silva, Rodriguez de Fonseca, Banin, Aledo, Baixeras, Sanz, Cuesta-Muñoz: "Opposite clinical phenotypes of glucokinase disease: Description of a novel activating mutation and contiguous inactivating mutations in human glucokinase (GCK) gene." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 12, pp. 1983-9, (2009) (PubMed).

Yoshida, Kato, Yokoi, Oguri, Watanabe, Metoki, Yoshida, Satoh, Aoyagi, Nishigaki, Nozawa, Yamada: "Association of genetic variants with chronic kidney disease in individuals with different lipid profiles." in: International journal of molecular medicine, Vol. 24, Issue 2, pp. 233-46, (2009) (PubMed).

Details zu GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Andere Bezeichnung: GCK

Hintergrund: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Synonyms: GK, GLK, HK4, HHF3, HKIV, HXKP, LGLK, MODY2, FGQTL3

Molekulargewicht: 52kDa

Gen-ID: 2645

Pathways: MAPK Signalweg, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process