Exonuclease 1 Antikörper (N-Term)

Produktnummer ABIN966108

Produktdetails anti-Exonuclease 1 Antikörper

Target: Exonuclease 1 (EXO1)

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Exonuclease 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Aufreinigung: Purified by antigen-specific affinity chromatography.

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human exonuclease 1

Anwendungsinformationen

Applikationshinweise: ELISA, Western blotting: 1µg/ml for 2hrs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: This antibody is stored in PBS, 50% glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Exonuclease 1 Antikörper (ABIN966108)

Genschel, Modrich: "Functions of MutLalpha, replication protein A (RPA), and HMGB1 in 5'-directed mismatch repair." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21536-44, (2009) (PubMed).

Sharma, Sommers, Driscoll, Uzdilla, Wilson, Brosh: "The exonucleolytic and endonucleolytic cleavage activities of human exonuclease 1 are stimulated by an interaction with the carboxyl-terminal region of the Werner syndrome protein." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23487-96, (2003) (PubMed).

Details zu Exonuclease 1

Target: Exonuclease 1 (EXO1)

Andere Bezeichnung: EXO1 (EXO1 Produkte)

Synonyme: HEX1 antikoerper, hExoI antikoerper, 5730442G03Rik antikoerper, Msa antikoerper, EXO1 antikoerper, exoi antikoerper, fc16e04 antikoerper, wu:fc16e04 antikoerper, zgc:55521 antikoerper, exonuclease 1 antikoerper, hypothetical protein antikoerper, exonuclease I Exo1 antikoerper, RNA exonuclease antikoerper, exonuclease 1 S homeolog antikoerper, EXO1 antikoerper, Y17G7B.12 antikoerper, SJAG_01832 antikoerper, PAS_chr1-1_0377 antikoerper, Exo1 antikoerper, exo1 antikoerper, exo1.S antikoerper, LOC100338764 antikoerper

Hintergrund: EXO1 (exonuclease 1) is a 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatchcontaining DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5' overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5' end of a downstream Okazaki fragment. EXO1 is required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. It is essential for male and female meiosis. EXO1 interacts with the MLH1-PMS2 heterodimer via MLH1. It interacts with MSH3 and with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. EXO1 interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. EXO1 interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5' overhanging flap structures. It also interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5' overhanging flap structures.

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response