alpha 2 Macroglobulin Antikörper (C-Term)

Produktnummer ABIN965492

Der Kaninchen Polyklonal Anti-alpha 2 Macroglobulin-Antikörper wurde für IHC validiert. Er ist geeignet, alpha 2 Macroglobulin in Proben von Human und Ratte zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für alpha 2 Macroglobulin Antikörper (C-Term) (ABIN965492)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha 2 Macroglobulin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Produktdetails anti-alpha 2 Macroglobulin Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Purified by antigen-specific affinity chromatography.

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human A2M (Alpha-2-macroglobulin precursor)

Anwendungsinformationen

Applikationshinweise: ELISA, Western blotting: 1µg/ml for 2hrs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: This antibody is stored in PBS, 50% glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-alpha 2 Macroglobulin Antikörper (ABIN965492)

Zhang, Li, Martin, Aebersold: "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." in: Nature biotechnology, Vol. 21, Issue 6, pp. 660-6, (2003) (PubMed).

Matthijs, Devriendt, Cassiman, Van den Berghe, Marynen: "Structure of the human alpha-2 macroglobulin gene and its promotor." in: Biochemical and biophysical research communications, Vol. 184, Issue 2, pp. 596-603, (1992) (PubMed).

Bishop, Bell: "Assembly of the endoplasmic reticulum phospholipid bilayer: the phosphatidylcholine transporter." in: Cell, Vol. 42, Issue 1, pp. 51-60, (1985) (PubMed).

Sottrup-Jensen, Stepanik, Kristensen, Wierzbicki, Jones, Lønblad, Magnusson, Petersen: "Primary structure of human alpha 2-macroglobulin. V. The complete structure." in: The Journal of biological chemistry, Vol. 259, Issue 13, pp. 8318-27, (1984) (PubMed).

Sottrup-Jensen, Lønblad, Stepanik, Petersen, Magnusson, Jörnvall: "Primary structure of the 'bait' region for proteinases in alpha 2-macroglobulin. Nature of the complex." in: FEBS letters, Vol. 127, Issue 2, pp. 167-73, (1981) (PubMed).

Details zu alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Andere Bezeichnung: A2M

Hintergrund: A2M (Alpha-2-macroglobulin) is able to inhibit all four classes of proteinases by a unique `trapping' mechanism. This protein has a peptide stretch, called the `bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thiolester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Pathways: Lipid Metabolism