VEGF Antikörper (Vascular Endothelial Growth Factor) (HRP)

Details for Product anti-VEGF Antibody No. ABIN964773
Antigen
  • MVCD1
  • VEGF
  • VPF
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF-A
  • VEGF164
  • eVEGF120
  • eVEGF164
  • vegf-a
  • vegf
  • vascular endothelial growth factor A
  • vascular endothelial growth factor A L homeolog
  • vascular endothelial growth factor
  • vascular endothelial growth factor precursor
  • VEGFA
  • Vegfa
  • vegfa.L
  • VEGF
  • vegf
Reaktivität
Human
160
69
59
15
4
2
2
2
2
1
Wirt
Kaninchen
128
51
20
4
4
2
2
Klonalität
Polyklonal
Konjugat
Dieser VEGF Antikörper ist konjugiert mit HRP
30
9
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
1
1
Applikation
ELISA, Western Blotting (WB)
153
119
43
41
26
22
18
14
10
8
5
4
3
3
3
2
1
1
1
1
1
Optionen
Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Immunogen Type: RecombinantProtein
Isotyp IgG
Spezifität This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Produktmerkmale VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K -> N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
Reinigung purified
Antigen
Andere Bezeichnung VEGF (VEGF Antibody Abstract)
Hintergrund VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
Synonyms: Vascular endothelial growth factor A; VEGF-A; VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor; VPF
Gen-ID 7422
NCBI Accession NP_001165097
Applikations-hinweise This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
Kommentare

Gene Name: VEGFA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungs-mittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide!
Lagerung 4 °C
Informationen zur Lagerung Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for anti-Vascular Endothelial Growth Factor (VEGF) antibody (HRP) (ABIN964773) Western Blot showing detection of Recombinant Human VEGF-165. 50ng of protein (Lane 1...
Produkt verwendet in: Sreevalsan, Safe: "The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells." in: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2483-93, 2014 (PubMed).

Haben Sie etwas anderes gesucht?