NEDD1 Antikörper

Produktnummer ABIN964675

Hersteller Produkt- Nr.: 109-401-c38

Produktdetails anti-NEDD1 Antikörper

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 109-401-c38

Hersteller: Rockland

Verwendungszweck: NEDD1 Antibody

Kreuzreaktivität (Details): This antibody reacts with endogenous Nedd1 protein.

Produktmerkmale: Synonyms: GCP-WD, Neural precursor cell expressed developmentally down-regulated protein 1, rabbit anti-NEDD1 Antibody

Aufreinigung: This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kDa in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:10,000

ELISA Dilution: 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 40 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NEDD1

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Andere Bezeichnung: NEDD1 (NEDD1 Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1, also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex. Gamma-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the gamma-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.

Gen-ID: 121441

NCBI Accession: NP_001128647

Pathways: M Phase