GST Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN964665

Hersteller Produkt- Nr.: 600-405-200

Dieses Anti-GST-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GST in WB, ELISA und IHC. Geeignet für Schistosoma japonicum.

Kurzübersicht für GST Antikörper (Alkaline Phosphatase (AP)) (ABIN964665)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-GST Antikörper (Alkaline Phosphatase (AP))

Hersteller Produkt- Nr.: 600-405-200

Hersteller: Rockland

Verwendungszweck: GST Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Produktmerkmale: Synonyms: rabbit anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200-1:1,000

Application Note: Anti-Glutathione-S-Transferase Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500-1:2,500

ELISA Dilution: 1:2,000-1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST

Hintergrund: Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.