BDNF Antikörper

Produktnummer ABIN964574

Produktdetails anti-BDNF Antikörper

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BDNF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is specific for human BDNF protein. A BLAST analysis was used to suggest cross-reactivity with BDNF from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to BDNF from a wide variety of animals. Cross-reactivity with BDNF from other sources has not been determined.

Produktmerkmale: BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum, it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Aufreinigung: affinity purified

Sterilität: Sterile filtered

Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E.coli.
Immunogen Type: RecombinantProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.

Kommentare:

Gene Name: BDNF

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.

Haltbarkeit: 6 months

Details zu BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF (BDNF Produkte)

Synonyme: ANON2 antikoerper, BULN2 antikoerper, bdnf-A antikoerper, BDNF antikoerper, bdnf antikoerper, brain derived neurotrophic factor antikoerper, brain-derived neurotrophic factor antikoerper, brain-derived neurotrophic factor L homeolog antikoerper, brain-derived neurotrophic factor S homeolog antikoerper, BDNF antikoerper, Bdnf antikoerper, bdnf antikoerper, bdnf.L antikoerper, bdnf.S antikoerper

Hintergrund: BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum; it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Synonyms: Brain-derived neurotrophic factor, Abrineurin cytokine

Gen-ID: 627

UniProt: P23560

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity