ATG13 Antikörper (pSer318)

Produktnummer ABIN964569

Hersteller Produkt- Nr.: 600-401-c49

Dieses Anti-ATG13-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG13 in WB, ELISA, DB und FACS. Geeignet für Human. Dieses Primary Antibody wurde in 20+ Publikationen zitiert.

Kurzübersicht für ATG13 Antikörper (pSer318) (ABIN964569)

Target: ATG13 (Autophagy Related 13 (ATG13))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Flow Cytometry (FACS)

Produktdetails anti-ATG13 Antikörper

Bindungsspezifität: pSer318

Hersteller Produkt- Nr.: 600-401-c49

Hersteller: Rockland

Verwendungszweck: ATG13 phospho S318 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue.

Produktmerkmale: Synonyms: rabbit anti-ATG13 pS318 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-ATG13 pS318 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. 

Western Blot Dilution: 1:2000

ELISA Dilution: 1:25,000-1:175,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ATG13 Antikörper (ABIN964569)

Jia, Bonifacino: "Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3." in: eLife, Vol. 8, (2020) (PubMed).

Izumi, Li, Shibaki, Mori, Yasunami, Sato, Matsunaga, Mae, Kodama, Kamijo, Kaneko, Nakagawara: "Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region." in: Scientific reports, Vol. 9, Issue 1, pp. 2236, (2019) (PubMed).

Wang, Maxwell, Joo, Gwon, Messing, Mishra, Shaw, Ward, Quan, Sakurada, Pruett-Miller, Bertorini, Vogel, Kim, Peng, Taylor, Kundu: "ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97." in: Molecular cell, Vol. 74, Issue 4, pp. 742-757.e8, (2019) (PubMed).

Wallot-Hieke, Verma, Schlütermann, Berleth, Deitersen, Böhler, Stuhldreier, Wu, Seggewiß, Peter, Gohlke, Mizushima, Stork: "Systematic analysis of ATG13 domain requirements for autophagy induction." in: Autophagy, Vol. 14, Issue 5, pp. 743-763, (2019) (PubMed).

Thayer, Awad, Hegdekar, Sarkar, Tesfay, Burt, Zeng, Feldman, Lipinski: "The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability." in: Autophagy, pp. 1-14, (2019) (PubMed).

Fuqua, Mere, Kronemberger, Blomme, Bae, Turner, Harris, Scudese, Edwards, Ebert, de Sousa, Bodine, Yang, Adams, Lira: "ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, Issue 11, pp. 11735-11745, (2019) (PubMed).

Jeong, Simoneschi, Keegan, Melville, Adler, Saraf, Florens, Washburn, Cavasotto, Fenyö, Cuervo, Rossi, Pagano: "The ULK1-FBXW5-SEC23B nexus controls autophagy." in: eLife, Vol. 7, (2019) (PubMed).

Wang, Wang, Zhang, Luo, Liu, Xu, Diao, Liao, Liu: "Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy." in: Nature communications, Vol. 9, Issue 1, pp. 3492, (2018) (PubMed).

Baron, Boudi, Dias, Schilling, Nölle, Vizcay-Barrena, Rattray, Jungbluth, Scheper, Fleck, Bates, Fanto: "Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration." in: Current biology : CB, Vol. 27, Issue 23, pp. 3626-3642.e6, (2018) (PubMed).

Jung, Nayak, Schaeffer, Starzetz, Kirsch, Müller, Dikic, Mittelbronn, Behrends: "Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator." in: eLife, Vol. 6, (2017) (PubMed).

Hieke, Löffler, Kaizuka, Berleth, Böhler, Drießen, Stuhldreier, Friesen, Assani, Schmitz, Peter, Diedrich, Dengjel, Holland, Simonsen, Wesselborg, Mizushima, Stork: "Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells." in: Autophagy, Vol. 11, Issue 9, pp. 1471-83, (2016) (PubMed).

Puente, Hendrickson, Jiang: "Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy." in: The Journal of biological chemistry, Vol. 291, Issue 11, pp. 6026-35, (2016) (PubMed).

Park, Jung, Seo, Otto, Grunwald, Kim, Moriarity, Kim, Starker, Nho, Voytas, Kim: "The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14." in: Autophagy, Vol. 12, Issue 3, pp. 547-64, (2016) (PubMed).

Jiao, Su, Dong, Zhang, Xie, Yao, Chen, Wang, Liou, You: "Chaperone-like protein p32 regulates ULK1 stability and autophagy." in: Cell death and differentiation, Vol. 22, Issue 11, pp. 1812-23, (2016) (PubMed).

Rosenberg, Lafitte, Grant, Chen, Cleveland, Duckett: "Development of an HTS-Compatible Assay for the Discovery of Ulk1 Inhibitors." in: Journal of biomolecular screening, Vol. 20, Issue 7, pp. 913-20, (2016) (PubMed).

Egan, Chun, Vamos, Zou, Rong, Miller, Lou, Raveendra-Panickar, Yang, Sheffler, Teriete, Asara, Turk, Cosford, Shaw: "Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates." in: Molecular cell, Vol. 59, Issue 2, pp. 285-97, (2015) (PubMed).

Puustinen, Rytter, Mortensen, Kohonen, Moreira, Jäättelä: "CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation." in: The Journal of cell biology, Vol. 204, Issue 5, pp. 713-27, (2014) (PubMed).

Stanton, Dutta, Zhang, Polavaram, Leontovich, Hönscheid, Sinicrope, Tindall, Muders, Datta: "Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance." in: Cancer research, Vol. 73, Issue 1, pp. 160-71, (2013) (PubMed).

Nazio, Strappazzon, Antonioli, Bielli, Cianfanelli, Bordi, Gretzmeier, Dengjel, Piacentini, Fimia, Cecconi: "mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6." in: Nature cell biology, Vol. 15, Issue 4, pp. 406-16, (2013) (PubMed).

Joo, Dorsey, Joshi, Hennessy-Walters, Rose, McCastlain, Zhang, Iyengar, Jung, Suen, Steeves, Yang, Prater, Kim, Thompson, Youle, Ney, Cleveland, Kundu: "Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy." in: Molecular cell, Vol. 43, Issue 4, pp. 572-85, (2011) (PubMed).

Details zu ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Andere Bezeichnung: ATG13

Hintergrund: Background: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.

Gen-ID: 9776

NCBI Accession: NP_001136145

UniProt: O75143

Pathways: PI3K-Akt Signalweg, Autophagie