Antipeptide antibodies were generated in chickens against two sequences shared between the Mouse (P10637.3) and Human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.2 mg/mL (0.1 mg/mL of each antibody)
Buffer
10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted in the dark at 2-8 °C.
Target
MAPT
(Microtubule-Associated Protein tau (MAPT))
Andere Bezeichnung
MAPT / tau
Hintergrund
Human Tau protein (a.k.a., Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau