DYKDDDDK Tag Antikörper

Produktnummer ABIN952003

Dieser Huhn Polyklonal Antikörper detektiert spezifisch DYKDDDDK Tag in WB, IP, IF, EIA und IHC (fro).

Kurzübersicht für DYKDDDDK Tag Antikörper (ABIN952003)

Target: DYKDDDDK Tag

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-DYKDDDDK Tag Antikörper

Sequenz: DYKDDDDK

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide containing the DYKDDDDK epitope conjugated to Keyhole Limpet Hemocyanin (KLH). Production: The first injection included complete Freund’s adjuvant, subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against Phosphate-Buffered isotonic Saline. Sodium azide was added, the protein concentration was adjusted and the antibodies were filter-sterilized (0.2 mym).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL (based on absorbance at 280 nm)

Buffer: PBS, pH 7.2 containing 0.02 % (w/v) Sodium Azide as an anti-microbial agent.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted in the dark at 2-8 °C.

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Andere Bezeichnung: DYKDDDDK Epitope Tag

Substanzklasse: Tag

Hintergrund: Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag