CD3 Antikörper (Biotin)

Produktnummer ABIN951289

Produktdetails anti-CD3 Antikörper (Biotin)

Target: CD3

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Spezifität: This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transduction. When immobilized this antibody can be used to stimulate mouse T cells in vitro1. A combination of this antibody and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.

Klon: 500-A2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protein to bring the total protein concentration to 4-5 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Should this product contain a precipitate we recommend microcentrifugation before use.

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Hintergrund: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gen-ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway