Kinesin (heavy chain) Antikörper

Produktnummer ABIN94412

Der Maus Monoklonal Anti--Antikörper wurde für ICC validiert. Er ist geeignet, in Proben von Human, Maus, Schwein und Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Kinesin (heavy chain) Antikörper (ABIN94412)

Target: Kinesin (heavy chain)

Reaktivität: Human, Maus, Schwein, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC)

Klon: KN-02

Produktdetails

Verwendungszweck: Anti-Kinesin Heavy Chain Purified

Spezifität: The antibody KN-02 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Kreuzreaktivität (Details): Mouse, Human, Rat, Other not tested, Porcine

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Enriched fraction of porcine brain kinesin.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, (2005) (PubMed).

Mac?rek, Dráberová, Richterová, Böhm, Dráber: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, (2003) (PubMed).

Antigendetails

Target: Kinesin (heavy chain)

Andere Bezeichnung: Kinesin Heavy Chain

Hintergrund: Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KHC, KINHC, KIF5

Pathways: Peptide Hormone Metabolism