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GFAP Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GFAP in WB, ICC, IHC (p) und IP. Er zeigt eine Reaktivität gegenüber Human, Schwein und Cat und wurde in 1 Publikation erwähnt.
Produktnummer ABIN94318

Kurzübersicht für GFAP Antikörper (ABIN94318)

Target

Alle GFAP Antikörper anzeigen
GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität

  • 308
  • 194
  • 192
  • 54
  • 50
  • 34
  • 32
  • 12
  • 11
  • 9
  • 7
  • 6
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Schwein, Cat

Wirt

  • 220
  • 176
  • 13
  • 2
  • 2
  • 2
Maus

Klonalität

  • 209
  • 203
  • 2
Monoklonal

Konjugat

  • 223
  • 36
  • 25
  • 17
  • 11
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Dieser GFAP Antikörper ist unkonjugiert

Applikation

  • 329
  • 189
  • 111
  • 100
  • 89
  • 80
  • 68
  • 42
  • 26
  • 26
  • 23
  • 17
  • 13
  • 9
  • 8
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon

GF-01
  • Verwendungszweck

    Anti-GFAP Purified

    Spezifität

    The antibody GF-01 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.

    Keine Kreuzreaktivität

    Ratte

    Kreuzreaktivität (Details)

    Human, Feline (Cat), Porcine

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.

    Isotyp

    IgG1
  • Applikationshinweise

    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: human brain (cortex, cerebellum). The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues.
    Immunocytochemistry: Recommended dilution: 5-10 μg/mL.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Lukás, Dráber, Bucek, Dráberová, Viklický, Stasková: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, (1990) (PubMed).

  • Target

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    Andere Bezeichnung

    GFAP

    Hintergrund

    Glial fibrillary acidic proteinprovided,GFAP (glial fibrillary acidic protein) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’,s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.,GFAP, ALXDRD

    Gen-ID

    2670

    UniProt

    P14136
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