TERT Antikörper (AA 597-611)

Produktnummer ABIN940568

Der Ziege Polyklonal Anti-TERT-Antikörper wurde für ELISA, FACS und IF validiert. Er ist geeignet, TERT in Proben von Human zu detektieren.

Kurzübersicht für TERT Antikörper (AA 597-611) (ABIN940568)

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TERT Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-TERT Antikörper

Bindungsspezifität: AA 597-611

Verwendungszweck: TERT (aa597-611)

Sequenz: QLRELSEAEV RQHRE

Spezifität: This antibody is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2, NP_001180305.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 125 kDa in lysates of cell lines A549 and CaCo-2 after 1 μg/mL antibody staining (calculated MW of 127 kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Andere Bezeichnung: TERT

Hintergrund: TERT, telomerase reverse transcriptase, EST2, TCS1, TP2, TRT, hEST2, telomerase catalytic subunit, telomerase-associated protein 2

Gen-ID: 7015

NCBI Accession: NP_937983, NP_001180305

Pathways: Telomere Maintenance