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ITGAL Antikörper (FITC)

This anti-ITGAL antibody (ABIN93951) is a Mouse Monoclonal antibody detecting ITGAL in FACS. Suitable for Human.
Produktnummer ABIN93951

Kurzübersicht für ITGAL Antikörper (FITC) (ABIN93951)

Target

Alle ITGAL Antikörper anzeigen
ITGAL (Integrin alpha-L (ITGAL))

Reaktivität

  • 149
  • 78
  • 20
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 117
  • 62
  • 54
  • 1
Maus

Klonalität

  • 183
  • 50
  • 1
Monoklonal

Konjugat

  • 92
  • 28
  • 23
  • 20
  • 10
  • 8
  • 6
  • 6
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ITGAL Antikörper ist konjugiert mit FITC

Applikation

  • 161
  • 57
  • 53
  • 46
  • 35
  • 31
  • 23
  • 19
  • 19
  • 7
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Klon

MEM-25
  • Verwendungszweck

    Anti-Hu CD11a FITC

    Spezifität

    The antibody MEM-25 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

    Kreuzreaktivität (Details)

    Human

    Aufreinigung

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    Leukocytes from a patient suffering from a LGL-type leukaemia.

    Isotyp

    IgG1
  • Applikationshinweise

    Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    No reconstitution is necessary.

    Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.
    Avoid prolonged exposure to light.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Lesport, Baudhuin, Sousa, Lemaoult, Zamborlini, Rouas-Freiss, Carosella, Favier: "Inhibition of human Vγ9Vδ2 T-cell antitumoral activity through HLA-G: implications for immunotherapy of cancer." in: Cellular and molecular life sciences : CMLS, (2011) (PubMed).

    Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).

    Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, (2009) (PubMed).

    Ottonello, Epstein, Mancini, Dapino, Dallegri: "Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G." in: Journal of leukocyte biology, Vol. 75, Issue 1, pp. 99-105, (2004) (PubMed).

    Mathison, Befus, Davison, Woodman: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, (2003) (PubMed).

    Hajishengallis, Martin, Sojar, Sharma, Schifferle, DeNardin, Russell, Genco: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, (2002) (PubMed).

    Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, (2000) (PubMed).

    Simon, Cherapanov, Nadra, Waddell, Seo, Wang, Doerschuk, Downey: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, (1999) (PubMed).

    Bazil, Stefanová, Hilgert, Kristofová, Van?k, Horejsí: "Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein." in: Folia biologica, Vol. 36, Issue 1, pp. 41-50, (1990) (PubMed).

  • Target

    ITGAL (Integrin alpha-L (ITGAL))

    Andere Bezeichnung

    CD11a

    Hintergrund

    Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL

    Gen-ID

    3683

    UniProt

    P20701

    Pathways

    Activated T Cell Proliferation, Integrin Complex
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