UBA3 Antikörper (N-Term)

Produktnummer ABIN928369

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch UBA3 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Zebrafisch (Danio rerio), Rind (Kuh), Hund, Huhn und Xenopus laevis.

Kurzübersicht für UBA3 Antikörper (N-Term) (ABIN928369)

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Rind (Kuh), Hund, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-UBA3 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Maus, Ratte (Rattus), Rind (Kuh), Hund, Huhn, Frosch, Zebrafisch (Brachydanio rerio)

Aufreinigung: Purified

Immunogen: UBA3 antibody was raised in rabbit using the N terminal of UBA3 as the immunogen

Anwendungsinformationen

Applikationshinweise: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Kommentare:

UBA3 Blocking Peptide, (ABIN937832), is also available for use as a blocking control in assays to test for specificity of this UBA3 antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Details zu UBA3

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Andere Bezeichnung: UBA3

Hintergrund: UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Synonyms: Polyclonal UBA3 antibody, Anti-UBA3 antibody, ubiquitin-like modifier activating enzyme 3 antibody, DKFZp566J164 antibody, MGC22384 antibody, UBE1C antibody, hUBA3 antibody.