UBA3 Antikörper (N-Term)

Produktnummer ABIN2784205

Der Kaninchen Polyklonal anti-UBA3 Antikörper wird verwendet zum Nachweis von UBA3 in Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio), Rind (Kuh), Hund, Pferd, Schwein und Kaninchen. Er wurde validiert für WB und IHC.

Kurzübersicht für UBA3 Antikörper (N-Term) (ABIN2784205)

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Rind (Kuh), Hund, Pferd, Schwein, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-UBA3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: UBA3 Antibody - N-terminal region

Sequenz: EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV IGAGGLGCEL

Homologie: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Produktmerkmale: This is a rabbit polyclonal antibody against UBA3. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50493-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu UBA3

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Andere Bezeichnung: UBA3

Hintergrund:

Background Information: UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: Ubiquitin-like modifier activating enzyme 3

Alternative Symbols: NAE2, UBE1C, hUBA3

Protein Name: NEDD8-activating enzyme E1 catalytic subunit

Molekulargewicht: 52kDa

Gen-ID: 9039

NCBI Accession: NP_003959

UniProt: Q8TBC4