P-Glycoprotein Antikörper

Produktnummer ABIN920703

Dieses Anti-P-Glycoprotein-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von P-Glycoprotein in WB, IHC, FACS und ICC. Geeignet für Human und Hamster.

Kurzübersicht für P-Glycoprotein Antikörper (ABIN920703)

Target: P-Glycoprotein

Reaktivität: Human, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P-Glycoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: JSB-1

Produktdetails anti-P-Glycoprotein Antikörper

Spezifität: JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Can be used for the detection of MDR cells in human tumor samples. Flow Cytometry: Use at least 1:10. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse-FITC. Immunocytochemistry: Use at least 1:20 (acetone fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed paraffin embedded tissues: Use at 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used, paraffin-embedded tissue can also be stained with the antibody. Western blotting: Start optimizing working dilution at 1:10, followed by incubation with anti-mouse HRP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 1 ml concentrated tissue culture supernatant (200 tests) at ~250 µg IgG/ml, with 1% BSA and 0.1% NaN3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu P-Glycoprotein

Target: P-Glycoprotein