P-Glycoprotein Antikörper

Details zu Produkt Nr. ABIN967562, Anbieter: Anmelden zum Anzeigen
Antigen
  • ABC20
  • CD243
  • CLCS
  • GP170
  • MDR1
  • P-GP
  • PGY1
  • Abcb1
  • Mdr1a
  • ABCB1
  • ABCB4
  • CMDR1
  • xemdr
  • ATP binding cassette subfamily B member 1
  • ATP binding cassette subfamily B member 1A
  • ATP binding cassette subfamily B member 1 L homeolog
  • ABCB1
  • Abcb1a
  • abcb1.L
Reaktivität
Human
25
4
4
2
1
Wirt
Maus
16
10
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
17
8
7
6
6
5
2
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Pharmingen™
Klon 17F9
Isotyp IgG2b
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Hintergrund Reacts with a 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody binds to the surface of the viable, unfixed MDR+ cell line, PMG-Y (please inquire), and is able to partially block the binding of UIC2 antibody (another MDR-specific monoclonal antibody). Immunoprecipitation application is reported, but not routinely tested in house.
Synonyms: MDR
Applikationshinweise Positive control cells are available through special order
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-P-Glycoprotein antibody (ABIN967562) Profile of P-glycoprotein (MDR) expressed on PMG-Y cell line analyzed on a FACScan (B...
Produkt verwendet in: Shi, Wrin, Reeder, Liu, Ring: "High-affinity monoclonal antibodies against P-glycoprotein." in: Clinical immunology and immunopathology, Vol. 76, Issue 1 Pt 1, pp. 44-51, 1995 (PubMed).

Bénard, Bourhis, Riou: "Clinical significance of multiple drug resistance in human cancers." in: Anticancer research, Vol. 10, Issue 5A, pp. 1297-302, 1990 (PubMed).

Goldstein, Galski, Fojo, Willingham, Lai, Gazdar, Pirker, Green, Crist, Brodeur: "Expression of a multidrug resistance gene in human cancers." in: Journal of the National Cancer Institute, Vol. 81, Issue 2, pp. 116-24, 1989 (PubMed).

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