GFP Antikörper (AA 25-120) (AbBy Fluor® 647)

Produktnummer ABIN898640

Der Kaninchen Polyklonal Anti-GFP-Antikörper wurde für WB, IF (p) und IF (cc) validiert. Er ist geeignet, GFP in Proben von Aequorea victoria und Aequorea coerulescens (Belt Jellyfish) zu detektieren.

Kurzübersicht für GFP Antikörper (AA 25-120) (AbBy Fluor® 647) (ABIN898640)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit AbBy Fluor® 647

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Produktdetails anti-GFP Antikörper (AbBy Fluor® 647)

Bindungsspezifität: AA 25-120

Kreuzreaktivität: Schwein, Tag

Kreuzreaktivität (Details): Aequorea victoria, Aequorea coerulescens (belt jellyfish)

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund:

Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

UniProt: P42212