BID Antikörper (pSer61) (AbBy Fluor® 555)

Produktnummer ABIN876655

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BID in WB, FACS, IF (cc) und IF (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für BID Antikörper (pSer61) (AbBy Fluor® 555) (ABIN876655)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BID Antikörper ist konjugiert mit AbBy Fluor® 555

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-BID Antikörper (AbBy Fluor® 555)

Bindungsspezifität: pSer61

Kreuzreaktivität: Human

Homologie: Mouse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse Bid around the phosphorylation site of Ser61

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: Bid

Hintergrund:

Synonyms: Bid phospho S61, p-Bid phospho S61, BH3 interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist, AltName: BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID.

Background: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Gen-ID: 12122

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity