PLK1 Antikörper (pSer137) (AbBy Fluor® 647)

Produktnummer ABIN874239

Dieses Anti-PLK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PLK1 in WB, IF (cc) und IF (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für PLK1 Antikörper (pSer137) (AbBy Fluor® 647) (ABIN874239)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLK1 Antikörper ist konjugiert mit AbBy Fluor® 647

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-PLK1 Antikörper (AbBy Fluor® 647)

Bindungsspezifität: pSer137

Spezifität: This phosphorylation site is homologous across the listed species.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund:

Synonyms: PLK, STPK13, Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, PLK1

Background: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Phosphorylates NEDD1. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylates both ECT2 and RACGAP1, and thereby stimulates their interaction that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2.

Gen-ID: 5347

UniProt: P53350

Pathways: Zellzyklus, M Phase