CDK4 Antikörper (AA 295-299)

Produktnummer ABIN871659

Der Kaninchen Polyklonal anti-CDK4 Antikörper (ABIN871659) detektiert spezifisch CDK4 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CDK4 Antikörper (AA 295-299) (ABIN871659)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CDK4 Antikörper

Bindungsspezifität: AA 295-299

Spezifität: The antibody detects endogenous levels of total CDK4 protein.

Aufreinigung: Affinity purified

Immunogen: Peptide sequence around AA 295-299 (L-H-K-D-E) derived from Human CDK4. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Andere Bezeichnung: CDK4

Hintergrund: Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .

Molekulargewicht: 34 kDa

Gen-ID: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size