CDK4 Antikörper (C-Term)

Produktnummer ABIN1881714

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-CDK4 Antikörper (ABIN1881714) detektiert spezifisch CDK4 in WB, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für CDK4 Antikörper (C-Term) (ABIN1881714)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CDK4 Antikörper

Bindungsspezifität: AA 272-303, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Rat Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-303 amino acids from the C-terminal region of rat Cdk4.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:25. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-CDK4 Antikörper (ABIN1881714)

Cho, Phillips, Khan, Weaver: "Cloning of the rat cyclin-dependent kinase 4 cDNA: implication in proliferation-dependent expression in rat tissues." in: Biochemical and biophysical research communications, Vol. 191, Issue 3, pp. 860-5, (1993) (PubMed).

Details zu CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Andere Bezeichnung: Cdk4

Hintergrund: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Molekulargewicht: 33799

NCBI Accession: NP_446045

UniProt: P35426

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size