CD3 Antikörper

Produktnummer ABIN775704

Produktdetails anti-CD3 Antikörper

Highlights:

• High quality CD3 Primärantikörper for the detection of CD3.
• Reliable product with high standard validation data

Target: CD3

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Mass Spectrometry (MS)

Verwendungszweck: Anti-Hu CD3 Purified

Spezifität: The antibody UCHT1 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.

Kreuzreaktivität (Details): Human, Non-Human Primates

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: human thymocytes followed by Sezary T cells

Klon: UCHT1

Isotyp: IgG1 kappa

Produktspezifische Information:

What can the CD3 Antibody ABIN775704 be used for?: This monoclonal CD3 Antibody detects CD3. The CD3 Antibody has been validated for various applications and can be used for the detection of CD3 and derivatives by Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Frozen Sections), Immunocytochemistry, Mass Spectrometry.

What validation data is available for this CD3 Antibody?: The Primärantikörper is referenced in 9 publications and is characterized by a proven, very high reliability. It has currently 5 product images that show its performance in a variety of applications. The product is currently available in 100 μg quantity. CD3 Antibody for the detection of CD3 and derivatives.

What is the function of CD3?: The CD3 complex mediates signal transduction.

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry and immunohistochemistry (frozen sections): The epitope for UCHT1 is resistant to fixation.
Flow cytometry: Extracellular and intracellular staining, recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD3 Antikörper (ABIN775704)

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, (2005) (PubMed).

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, (2004) (PubMed).

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, (1998) (PubMed).

Garson, Beverley, Coakham, Harper: "Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species." in: Nature, Vol. 298, Issue 5872, pp. 375-7, (1982) (PubMed).

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Hintergrund: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway