POLA1 Antikörper (AA 1231-1350)

Produktnummer ABIN763391

Der Kaninchen Polyklonal Anti-POLA1-Antikörper wurde für WB, ELISA, IHC (fro), IF (cc), IF (p) und IHC (p) validiert. Er ist geeignet, POLA1 in Proben von Human zu detektieren.

Kurzübersicht für POLA1 Antikörper (AA 1231-1350) (ABIN763391)

Target: POLA1 (DNA Polymerase alpha (POLA1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POLA1 Antikörper

Bindungsspezifität: AA 1231-1350

Homologie: Human,Mouse,Rat,Dog,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DPOLA/DNA polymerase alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Andere Bezeichnung: Dna Polymerase alpha

Hintergrund:

Synonyms: DNA polymerase alpha 1 catalytic subunit, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DPOLA_HUMAN, P180, p180 subunit, POLA, POLA1, Polymerase DNA directed, alpha 1, catalytic subunit, Polymerase DNA-directed, alpha 70kD.

Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Gen-ID: 5422

Pathways: SARS-CoV-2 Protein Interaktom