POLA1 Antikörper (AA 1406-1439)

Produktnummer ABIN6243169

Der Kaninchen Polyklonal anti-POLA1 Antikörper (Klon RB57637) (ABIN6243169) detektiert spezifisch POLA1 in WB und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für POLA1 Antikörper (AA 1406-1439) (ABIN6243169)

Target: POLA1 (DNA Polymerase alpha (POLA1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: RB57637

Produktdetails anti-POLA1 Antikörper

Bindungsspezifität: AA 1406-1439

Homologie: H

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This POLA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1406-1439 amino acids from the human region of human POLA1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Andere Bezeichnung: POLA1

Hintergrund: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Molekulargewicht: 165913

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interaktom