MSK1 Antikörper (pSer360)

Produktnummer ABIN8124424

Der Kaninchen Monoklonal anti-MSK1 Antikörper (Klon AB1750) (ABIN8124424) detektiert spezifisch MSK1 in WB, ELISA, IF und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MSK1 Antikörper (pSer360) (ABIN8124424)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MSK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: AB1750

Produktdetails anti-MSK1 Antikörper

Bindungsspezifität: pSer360

Verwendungszweck: MSK1 (phospho Ser360) Rabbit mAb

Spezifität: The antibody detects endogenous levels of MSK1 protein only when phosphorylated at Ser360. The antibody does not cross-react with MSK1 phosphorylated at other sites.

Aufreinigung: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB, 1:1000-1:5000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at-20°C. Avoid freeze / thaw cycles.

Details zu MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Andere Bezeichnung: MSK1

Hintergrund:

Synonyms: RPS6KA5, MSK1, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen-and stress-activated protein kinase 1, RSK-like protein kinase, RSKL

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein. , cofactor:Magnesium. , enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process. , function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1) . Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) , which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen-and stress-induced phosphorylation of high mobility group protein 14 (HMG-14) . , miscellaneous:Enzyme activity requires the presence of both kinase domains. , PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. , similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. , similarity:Contains 1 AGC-kinase C-terminal domain. , similarity:Contains 2 protein kinase domains. , subcellular location:Predominantly nuclear. Partially cytoplasmic. , subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. , tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. ,

Gene Name: RPS6KA5

Protein Name: MSK1 (phospho Ser360)

Gen-ID: 9252

UniProt: O75582

Pathways: MAPK Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades