LAT Antikörper (pTyr255)

Produktnummer ABIN8122660

Der Kaninchen Polyklonal anti-LAT Antikörper (ABIN8122660) detektiert spezifisch LAT in WB, ELISA und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für LAT Antikörper (pTyr255) (ABIN8122660)

Target: LAT (Linker For Activation of T Cells (LAT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LAT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-LAT Antikörper

Bindungsspezifität: pTyr255

Verwendungszweck: LAT (Phospho Tyr255) Antibody

Spezifität: This antibody detects endogenous levels of Human,Mouse,Rat LAT (Phospho Tyr255)

Aufreinigung: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human LAT (Phospho Tyr255)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Andere Bezeichnung: LAT

Hintergrund:

Synonyms: Linker for activation of T-cells family member 1 (36 kDa phospho-tyrosine adapter protein, pp36, p36-38)

Background: function:Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.,miscellaneous:Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT.,PTM:Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.,PTM:Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation, which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement.,subcellular location:Present in lipid rafts.,subunit:When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A.,tissue specificity:Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).,

Gene Name: LAT

Protein Name: LAT (Phospho Tyr255)

Gen-ID: 27040

UniProt: O43561

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung