Cyclin E1 Antikörper

Produktnummer ABIN8120794

Der Kaninchen Monoklonal anti-Cyclin E1 Antikörper (Klon AB1742) (ABIN8120794) detektiert spezifisch Cyclin E1 in WB, IHC, ELISA, IF und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für Cyclin E1 Antikörper (ABIN8120794)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: AB1742

Produktdetails anti-Cyclin E1 Antikörper

Verwendungszweck: Cyclin E1 Rabbit mAb

Spezifität: This antibody detects endogenous levels of Cyclin E1 protein.

Aufreinigung: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB, 1:1000-1:5000 | IHC, 1:400-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at-20°C. Avoid freeze / thaw cycles.

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: Cyclin E1

Hintergrund:

Synonyms: CCNE1, CCNE, G1/S-specific cyclin-E1

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus) , which participates in

Gene Name: CCNE1

Protein Name: Cyclin E1

Gen-ID: 898

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases