Cyclin E1 Antikörper (AA 10-176)

Produktnummer ABIN6940994

Der Maus Monoklonal anti-Cyclin E1 Antikörper (Klon CCNE1-2460) (ABIN6940994) detektiert spezifisch Cyclin E1 in WB, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Cyclin E1 Antikörper (AA 10-176) (ABIN6940994)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: CCNE1-2460

Produktdetails anti-Cyclin E1 Antikörper

Bindungsspezifität: AA 10-176

Verwendungszweck: Cyclin E (G1/S-Phase Cyclin) Antibody

Spezifität: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, MCF, A431, HeLa cells. Breast or Colon Carcinoma.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: CCNE1

Hintergrund:

CCNE1, Cyclin E1, G1/S-specific cyclin-E1

Jurkat, HCT-116, MCF-7 or HeLa cells. Human breast or colon carcinoma.

Molekulargewicht: 53kDa

Gen-ID: 898, 244723

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases