Rekombinanter PLAP Antikörper (full length)

Produktnummer ABIN8093410

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch PLAP in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter PLAP Antikörper (full length) (ABIN8093410)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-350R

Produktdetails anti-PLAP Antikörper

Bindungsspezifität: full length

Verwendungszweck: ALPP Antibody / Alkaline phosphatase placental

Immunogen: Recombinant full-length human Alkaline phosphatase placental protein was used as the immunogen for the ALPP antibody for IHC.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the ALPP antibody for IHC should be determined by the researcher.

2. This ALPP/Alkaline phosphatase placental antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: ALPP/Alkaline phosphatase placental antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: ALPP

Hintergrund: Alkaline phosphatase placental is a glycosylphosphatidylinositol-anchored membrane enzyme encoded by the ALPP gene and commonly referred to as placental alkaline phosphatase or PLAP. The ALPP Antibody for IHC is developed to evaluate tissue distribution and membranous expression of this trophoblast-associated isoenzyme in formalin-fixed, paraffin-embedded specimens. ALPP is located on chromosome 2q37 and belongs to the alkaline phosphatase family of homodimeric metalloenzymes that hydrolyze phosphate monoesters under alkaline conditions.

In normal tissue, placental alkaline phosphatase is strongly expressed on the apical membrane of syncytiotrophoblasts, producing a distinct membranous staining pattern in placental tissue. Outside of pregnancy-associated tissues, ALPP expression is limited, which supports its utility in immunohistochemical panels assessing trophoblastic differentiation. The glycosylphosphatidylinositol anchor localizes the enzyme to the external surface of the plasma membrane, resulting in crisp membranous staining in positive epithelial or trophoblastic cells.

In tumor pathology research, ALPP is widely recognized as a marker of germ cell tumors, particularly seminoma and embryonal carcinoma, as well as certain trophoblastic neoplasms. In these contexts, strong membranous and occasionally cytoplasmic staining is observed in tumor epithelial cells, while most non-germ cell malignancies show minimal or absent staining. This restricted distribution pattern enhances its value in studies examining lineage differentiation and tumor classification within tissue sections.

Although ALPP shares structural similarity with other alkaline phosphatase isoenzymes, including tissue-nonspecific and intestinal alkaline phosphatases, its expression profile is more restricted and lineage-associated. Evaluation of ALPP expression by immunohistochemistry supports research focused on trophoblastic biology, germ cell tumor characterization, and membrane-associated enzyme localization within histologic architecture. Clone MSVA-350R is a recombinant rabbit monoclonal antibody developed for detection of ALPP in tissue-based research applications.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: P05187