Rekombinanter Perforin 1 Antikörper (AA 400-555)

Produktnummer ABIN8093400

Der Kaninchen Monoklonal anti-Perforin 1 Antikörper (Klon PRF1-9681R) (ABIN8093400) detektiert spezifisch Perforin 1 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter Perforin 1 Antikörper (AA 400-555) (ABIN8093400)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Perforin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Klon: PRF1-9681R

Produktdetails anti-Perforin 1 Antikörper

Bindungsspezifität: AA 400-555

Verwendungszweck: Perforin Antibody / PRF1 (azide and preservative free)

Immunogen: A recombinant fragment (around amino acids 400-555) of human Perforin-1 protein (exact sequence is proprietary) was used as the immunogen for the Cytotoxic Lymphocyte Perforin/PRF1 antibody.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the Cytotoxic Lymphocyte Perforin/PRF1 antibody should be determined by the researcher.

2. This Cytotoxic Lymphocyte Perforin/PRF1 antibody is recombinantly produced by expression in CHO cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Cytotoxic Lymphocyte Perforin/PRF1 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin

Hintergrund: Cytotoxic Lymphocyte Perforin Antibody recognizes Perforin, a calcium-dependent pore-forming protein encoded by the PRF1 gene. Perforin is a critical effector molecule of cytotoxic T lymphocytes and natural killer cells, where it is synthesized, stored in secretory granules, and released upon immune synapse formation. Following antigen recognition, Perforin is exocytosed and inserts into the membrane of target cells, forming transmembrane pores that allow granzymes to enter and initiate apoptosis. This tightly regulated mechanism is central to antiviral defense, tumor immunosurveillance, and elimination of intracellular pathogens.

Perforin belongs to the membrane attack complex/perforin (MACPF) protein family and contains a conserved MACPF domain responsible for pore formation. The protein also includes calcium-binding regions that regulate its conformational change and membrane insertion. Under resting conditions, Perforin remains sequestered within cytotoxic granules to prevent unintended tissue damage. Upon activation of cytotoxic lymphocytes, controlled secretion ensures targeted killing of infected or transformed cells while minimizing collateral injury to surrounding tissues.

The PRF1 gene is located on chromosome 10 and is predominantly expressed in activated CD8-positive T cells and natural killer cells. In lymphoid organs such as spleen, tonsil, and lymph node, Perforin-positive cells are typically scattered within germinal centers and interfollicular regions, reflecting the distribution of activated cytotoxic lymphocytes. Cytoplasmic granular staining is the expected localization pattern in immunohistochemistry due to storage within lytic granules.

Genetic mutations in PRF1 are associated with familial hemophagocytic lymphohistiocytosis and other immune dysregulation syndromes, highlighting the essential role of Perforin in maintaining immune homeostasis. Altered expression levels have also been studied in cancer immunology, transplant rejection, and autoimmune disease research. Quantifying Perforin expression can provide insight into cytotoxic immune activity within tumor microenvironments or inflammatory lesions.

This Cytotoxic Lymphocyte Perforin Antibody (clone PRF1/9681R) is a recombinant monoclonal antibody suitable for detecting PRF1 expression in research applications. A Cytotoxic Lymphocyte Perforin Antibody supports studies of cell-mediated cytotoxicity, immune activation status, and functional characterization of cytotoxic lymphocyte populations in normal and diseased tissues.

UniProt: P14222

Pathways: Apoptose, Caspase Kaskade in der Apoptose