Rekombinanter MSH2 Antikörper (full length)

Produktnummer ABIN8093331

Der Maus Monoklonal anti-MSH2 Antikörper (Klon MSVA-902M) (ABIN8093331) detektiert spezifisch MSH2 in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter MSH2 Antikörper (full length) (ABIN8093331)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-902M

Produktdetails anti-MSH2 Antikörper

Bindungsspezifität: full length

Verwendungszweck: MSH2 Antibody / MutS homolog 2

Immunogen: Recombinant full-length human MSH2 protein was used as the immunogen for the MSH2 / MutS homolog 2 antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the MSH2 Antibody for IHC / MutS homolog 2 Immunohistochemistry Antibody should be determined by the researcher.

2. This MSH2 / MutS homolog 2 antibody is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: MSH2 / MutS homolog 2 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2

Hintergrund: MutS homolog 2 (MSH2) is a nuclear DNA mismatch repair protein encoded by the MSH2 gene and is a central component of the cellular machinery responsible for maintaining genomic stability. MSH2 Antibody for IHC is designed for immunohistochemical detection of MutS homolog 2 / MSH2 in tissue sections, enabling visualization of nuclear expression patterns associated with DNA mismatch repair activity. MSH2 forms heterodimeric complexes with other mismatch repair proteins, including MSH6 and MSH3, allowing recognition and correction of base-base mismatches and insertion-deletion loops that occur during DNA replication. Because this repair pathway is essential for preserving genomic integrity, MSH2 expression is widely studied in cancer biology and tumor diagnostics.

MSH2 antibody, also referred to as MutS homolog 2 antibody or hMSH2 antibody in the literature, detects a nuclear protein that is broadly expressed in proliferating epithelial tissues and lymphoid cells. In immunohistochemistry applications, MSH2 staining typically appears as distinct nuclear labeling in cells with intact mismatch repair activity. MSH2 Antibody for IHC is therefore particularly useful for evaluating DNA mismatch repair status in tissue specimens, as loss of nuclear staining in tumor cells may indicate mismatch repair deficiency while surrounding non-neoplastic cells retain nuclear positivity and serve as internal controls.

Immunohistochemistry analysis using MSH2 Antibody for IHC plays a critical role in research and pathology studies examining mismatch repair deficiency and microsatellite instability. Loss of MSH2 expression has been strongly associated with hereditary cancer syndromes such as Lynch syndrome as well as a subset of sporadic colorectal, endometrial, and other carcinomas. Because of this biological significance, detection of MSH2 protein expression in formalin-fixed paraffin-embedded tissues is widely used to investigate tumor biology, genomic instability, and DNA repair mechanisms.

MSH2 Antibody for IHC (clone MSVA-902M) has been tested on many normal and cancer tissues, demonstrating nuclear immunohistochemical staining patterns that align with expression data reported in the Human Protein Atlas. This recombinant mouse monoclonal antibody provides a reliable reagent for immunohistochemistry studies evaluating mismatch repair protein expression, nuclear DNA repair pathways, and tumor-associated alterations in MSH2 expression.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response