ATF4 Antikörper (pSer245)

Produktnummer ABIN756172

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATF4 in WB, ELISA, IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für ATF4 Antikörper (pSer245) (ABIN756172)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ATF4 Antikörper

Bindungsspezifität: pSer245

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ATF4 around the phosphorylation site of Ser245

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-ATF4 Antikörper (ABIN756172)

Jiang, Yun, Shi, Niu, Gao, Xie, Yu: "Downregulation of miR-384-5p attenuates rotenone-induced neurotoxicity in dopaminergic SH-SY5Y cells through inhibiting endoplasmic reticulum stress." in: American journal of physiology. Cell physiology, Vol. 310, Issue 9, pp. C755-63, (2016) (PubMed).

Hayashi, Ouchi, Endou, Anzai: "HOXB9 acts as a negative regulator of activated human T cells in response to amino acid deficiency." in: Immunology and cell biology, Vol. 94, Issue 6, pp. 612-7, (2016) (PubMed).

Details zu ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Andere Bezeichnung: ATF4

Hintergrund:

Synonyms: ATF4 phospho S245, ATF4Ser245, Cyclic AMP response element binding protein 2, DNA binding protein TAXREB67, Activating Transcription Factor 4, ATF 4, ATF4 protein, CREB 2, CREB2, CREB-2, Cyclic AMP dependent transcription factor ATF 4, Tax Responsive Enhancer Element B67, TAXREB67, TXREB.

Background: ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Gen-ID: 468

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response