Topoisomerase II alpha Antikörper (C-Term)

Produktnummer ABIN8047566

Der Kaninchen Polyklonal anti-Topoisomerase II alpha Antikörper wird verwendet zum Nachweis von Topoisomerase II alpha in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für Topoisomerase II alpha Antikörper (C-Term) (ABIN8047566)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Güteklasse: Carrier-free

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-Topoisomerase II alpha/TOP2A Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: different from the related rat sequence by three amino acids,and different from the related mouse sequence by four amino acids.

Produktmerkmale: Anti-Topoisomerase II alpha/TOP2A Antibody. Tested in WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha, different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund:

Background: The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 Molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1 % of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.

Gene Full Name: DNA topoisomerase II alpha

Sequence Similarities: Belongs to the type II topoisomerase family.

Molekulargewicht: 174 kDa

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases