SPHK1 Antikörper (AA 55-357)

Produktnummer ABIN8041717

Der Kaninchen Polyklonal anti-SPHK1 Antikörper (ABIN8041717) detektiert spezifisch SPHK1 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für SPHK1 Antikörper (AA 55-357) (ABIN8041717)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Carrier-free

Produktdetails anti-SPHK1 Antikörper

Bindungsspezifität: AA 55-357

Verwendungszweck: Anti-SPHK1 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SPHK1 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SPHK1 recombinant protein (Position: E55-N357).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Andere Bezeichnung: SPHK1

Hintergrund:

Background: SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Gene Full Name: sphingosine kinase 1

Molekulargewicht: 52 kDa

Gen-ID: 8877

UniProt: Q9NYA1

Pathways: VEGF Signaling