RMI2 Antikörper (C-Term)

Produktnummer ABIN8033448

Der Kaninchen Polyklonal anti-RMI2 Antikörper wird verwendet zum Nachweis von RMI2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, FACS, IHC, ICC und IHC (fro).

Kurzübersicht für RMI2 Antikörper (C-Term) (ABIN8033448)

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RMI2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Güteklasse: Carrier-free

Produktdetails anti-RMI2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-RMI2 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-RMI2 Antibody. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RMI2, which shares 96.3% and 92.6% amino acid (aa) sequence identity with mouse and rat RMI2, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry, 0.5-1 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RMI2

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Andere Bezeichnung: RMI2

Hintergrund:

Background: RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (see RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted ly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90 % . Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.

Gene Full Name: RecQ mediated genome instability 2

Molekulargewicht: 19 kDa

Gen-ID: 116028

UniProt: Q96E14

Pathways: DNA Reparatur