MGMT Antikörper (AA 2-207) (HRP)

Produktnummer ABIN8017845

Der Kaninchen Polyklonal anti-MGMT Antikörper wird verwendet zum Nachweis von MGMT in Proben von Human. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für MGMT Antikörper (AA 2-207) (HRP) (ABIN8017845)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MGMT Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-MGMT Antikörper (HRP)

Bindungsspezifität: AA 2-207

Verwendungszweck: Anti-MGMT Antibody HRP Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human MGMT shares 70%,69% amino acid (aa) sequence identity with mouse,rat MGMT,respectively.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund:

Background: O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene. MGMT is mapped to 10q26.3. This gene is crucial for genome stability. It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival.

Gene Full Name: O-6-methylguanine-DNA methyltransferase

Sequence Similarities: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

Gen-ID: 4255

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus