MGMT Antikörper

Produktnummer ABIN7249814

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MGMT in IHC. Es zeigt Reaktivität gegenüber Proben von Ratte und Maus.

Kurzübersicht für MGMT Antikörper (ABIN7249814)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Produktdetails anti-MGMT Antikörper

Produktmerkmale: Polyclonal Antibody

Aufreinigung: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse MGMT

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 260 μg/mL

Buffer: PBS with 0.02 % sodium azide, 1 % BSA and 50 % glycerol, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.

UniProt: P26187, P24528

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus