MBD4 Antikörper (C-Term) (Fluoro488)

Produktnummer ABIN8009619

Der Kaninchen Polyklonal anti-MBD4 Antikörper wird verwendet zum Nachweis von MBD4 in Proben von Human, Maus und Ratte. Er wurde validiert für FACS.

Kurzübersicht für MBD4 Antikörper (C-Term) (Fluoro488) (ABIN8009619)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD4 Antikörper ist konjugiert mit Fluoro488

Applikation: Flow Cytometry (FACS)

Produktdetails anti-MBD4 Antikörper (Fluoro488)

Bindungsspezifität: C-Term

Verwendungszweck: Anti-MBD4 Antibody Fluoro488 Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: identical to the related rat,mouse sequences.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Andere Bezeichnung: MBD4

Hintergrund:

Background: MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

Gene Full Name: methyl-CpG binding domain 4, DNA glycosylase

Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.

Gen-ID: 8930

UniProt: O95243

Pathways: DNA Reparatur