MBD4 Antikörper (Middle Region)

Produktnummer ABIN2776028

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MBD4 in WB und ChIP. Es zeigt Reaktivität gegenüber Proben von Human und Meerschweinchen.

Kurzübersicht für MBD4 Antikörper (Middle Region) (ABIN2776028)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reaktivität: Human, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-MBD4 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: MBD4 Antibody - middle region

Sequenz: CSEQKTSGII NKFCSAKDSE HNEKYEDTFL ESEEIGTKVE VVERKEHLHT

Homologie: Guinea Pig: 83%, Human: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against MBD4. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MBD4

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34797-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Andere Bezeichnung: MBD4

Hintergrund:

Background Information: MBD4 is involved with DNA methylation. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD3 comprise a family of nuclear proteins related by??he presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Methyl-CpG binding domain protein 4

Alternative Symbols: MED1

Protein Name: Methyl-CpG-binding domain protein 4

Molekulargewicht: 66kDa

Gen-ID: 8930

NCBI Accession: NP_003916

UniProt: O95243

Pathways: DNA Reparatur