LIG4 Antikörper (AA 1-876)

Produktnummer ABIN8005996

Der Kaninchen Polyklonal anti-LIG4 Antikörper (ABIN8005996) detektiert spezifisch LIG4 in WB, ELISA, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für LIG4 Antikörper (AA 1-876) (ABIN8005996)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Produktdetails anti-LIG4 Antikörper

Bindungsspezifität: AA 1-876

Verwendungszweck: Anti-DNA Ligase IV/LIG4 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DNA Ligase IV/LIG4 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Andere Bezeichnung: LIG4

Hintergrund:

Background: DNA ligase 4 is an enzyme that in humans is encoded by the LIG4 gene. The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene Full Name: DNA ligase 4

Molekulargewicht: 104 kDa

Gen-ID: 3981

UniProt: P49917

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response