LIG4 Antikörper (AA 1-876) (Fluoro647)

Produktnummer ABIN8005995

Der Kaninchen Polyklonal anti-LIG4 Antikörper wird verwendet zum Nachweis von LIG4 in Proben von Human, Maus und Ratte. Er wurde validiert für FACS.

Kurzübersicht für LIG4 Antikörper (AA 1-876) (Fluoro647) (ABIN8005995)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG4 Antikörper ist konjugiert mit Fluoro647

Applikation: Flow Cytometry (FACS)

Produktdetails anti-LIG4 Antikörper (Fluoro647)

Bindungsspezifität: AA 1-876

Verwendungszweck: Anti-DNA Ligase IV/LIG4 Antibody Fluoro647 Conjugated

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Andere Bezeichnung: LIG4

Hintergrund:

Background: DNA ligase 4 is an enzyme that in humans is encoded by the LIG4 gene. The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene Full Name: DNA ligase 4

Gen-ID: 3981

UniProt: P49917

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response